df1 chicken embryonic fibroblast cell line  (Pasteur Institute)

Journal: Journal of Virology

Article Title: An LYPSL Late Domain in the Gag Protein Contributes to the Efficient Release and Replication of Rous Sarcoma Virus ▿

doi: 10.1128/JVI.00238-10

Figure Lengend Snippet: Effect of LYPSL mutations on the spread of infection. DF1 cells were transfected with proviral DNA harboring late domain mutations. At 1 day posttransfection, cells were diluted 1:10 with fresh cells, and infection was monitored at daily intervals by GFP detection via flow cytometry.

Article Snippet: The ev-0-derived chicken fibroblast cell line DF1 (ATCC CRL 12203) was grown in Dulbecco's modified Eagle medium (Invitrogen) supplemented with 5% fetal bovine serum (Gemini Bio-Products), 5% NuSerum (BD Biosciences), 2% heat-inactivated chicken serum (Invitrogen), 1% glutamine (Invitrogen), and 1% vitamins (Invitrogen).

Techniques: Infection, Transfection, Flow Cytometry

Journal: Journal of Virology

Article Title: An LYPSL Late Domain in the Gag Protein Contributes to the Efficient Release and Replication of Rous Sarcoma Virus ▿

doi: 10.1128/JVI.00238-10

Figure Lengend Snippet: Partial rescue of the budding and spreading rates of the PPPY mutants by the overexpression of human Alix. (A) Comparison of the budding rates of the wild type and late domain mutants in DF1 control cells (white bars) or DF1 cells overexpressing human Alix (black bars). The P value obtained from a Student's t test for the budding rates of the APPY-LYPSL mutant in DF1 cells and AlixDF1 cells was significant (P < 0.05). The P value for the AAAA-LYPSL mutant was unable to be calculated since the budding rate in DF1 cells is below the sensitivity of the assay, indicated by the dashed line. (B to E) Spreading profiles of the wild type (B) and the APPY-LYPSL (C), AAAA-LYPSL (D), and APPY-AAASA (E) mutants were generated by flow cytometry.

Article Snippet: The ev-0-derived chicken fibroblast cell line DF1 (ATCC CRL 12203) was grown in Dulbecco's modified Eagle medium (Invitrogen) supplemented with 5% fetal bovine serum (Gemini Bio-Products), 5% NuSerum (BD Biosciences), 2% heat-inactivated chicken serum (Invitrogen), 1% glutamine (Invitrogen), and 1% vitamins (Invitrogen).

Techniques: Over Expression, Comparison, Control, Mutagenesis, Generated, Flow Cytometry

Journal: Journal of Virology

Article Title: An LYPSL Late Domain in the Gag Protein Contributes to the Efficient Release and Replication of Rous Sarcoma Virus ▿

doi: 10.1128/JVI.00238-10

Figure Lengend Snippet: Comparison of budding and spreading rates in DF1 cells

Article Snippet: The ev-0-derived chicken fibroblast cell line DF1 (ATCC CRL 12203) was grown in Dulbecco's modified Eagle medium (Invitrogen) supplemented with 5% fetal bovine serum (Gemini Bio-Products), 5% NuSerum (BD Biosciences), 2% heat-inactivated chicken serum (Invitrogen), 1% glutamine (Invitrogen), and 1% vitamins (Invitrogen).

Techniques: Comparison, Mutagenesis

Journal: Journal of Virology

Article Title: An LYPSL Late Domain in the Gag Protein Contributes to the Efficient Release and Replication of Rous Sarcoma Virus ▿

doi: 10.1128/JVI.00238-10

Figure Lengend Snippet: The GFP intensity of infected cells is lower in cells infected with severe late domain mutants. The average fluorescence intensity of GFP-positive cells was determined as the virus spread through the population of DF1 cells.

Article Snippet: The ev-0-derived chicken fibroblast cell line DF1 (ATCC CRL 12203) was grown in Dulbecco's modified Eagle medium (Invitrogen) supplemented with 5% fetal bovine serum (Gemini Bio-Products), 5% NuSerum (BD Biosciences), 2% heat-inactivated chicken serum (Invitrogen), 1% glutamine (Invitrogen), and 1% vitamins (Invitrogen).

Techniques: Infection, Fluorescence, Virus

Journal: Journal of Virology

Article Title: An LYPSL Late Domain in the Gag Protein Contributes to the Efficient Release and Replication of Rous Sarcoma Virus ▿

doi: 10.1128/JVI.00238-10

Figure Lengend Snippet: Gag polyprotein cleavage in late domain virions. Gag proteins in wild-type and mutant virus pellets from control DF1 cells (−) or hAlix-DF1 cells were analyzed by immunoblotting with anti-CA rabbit serum.

Article Snippet: The ev-0-derived chicken fibroblast cell line DF1 (ATCC CRL 12203) was grown in Dulbecco's modified Eagle medium (Invitrogen) supplemented with 5% fetal bovine serum (Gemini Bio-Products), 5% NuSerum (BD Biosciences), 2% heat-inactivated chicken serum (Invitrogen), 1% glutamine (Invitrogen), and 1% vitamins (Invitrogen).

Techniques: Mutagenesis, Virus, Control, Western Blot

Journal: Journal of Virology

Article Title: An LYPSL Late Domain in the Gag Protein Contributes to the Efficient Release and Replication of Rous Sarcoma Virus ▿

doi: 10.1128/JVI.00238-10

Figure Lengend Snippet: Budding morphology of late domain mutants. Shown are representative scanning electron micrographs of DF1 cells transiently transfected with the wild type (A) and the PPPY-AAASA (B), APPY-AAASA (C), and AAAA-AAASA (D) mutants. The cells shown had similar levels of GFP fluorescence. The scale bars are 10 μm in the left images and 1 μm in the right images.

Article Snippet: The ev-0-derived chicken fibroblast cell line DF1 (ATCC CRL 12203) was grown in Dulbecco's modified Eagle medium (Invitrogen) supplemented with 5% fetal bovine serum (Gemini Bio-Products), 5% NuSerum (BD Biosciences), 2% heat-inactivated chicken serum (Invitrogen), 1% glutamine (Invitrogen), and 1% vitamins (Invitrogen).

Techniques: Transfection, Fluorescence